Journal: Oncology Letters

Article Title: Schisandrin B suppresses cholangiocarcinoma by targeting the ROS/p38 MAPK/NF-κB axis

doi: 10.3892/ol.2026.15551

Figure Lengend Snippet: Sch B inhibits NF-κB signaling pathway expression. mRNA expression levels of (A) IL-6, (B) IL-8 (C) and TNF-α were measured in CCA cells treated with different concentrations of Sch B (0, 40, 80, 160 µmol/l). Statistical analysis was performed using Welch's ANOVA followed by Dunnett's T3 post hoc test. (D) NF-κB expression in CCA cells was evaluated using a double luciferase assay with different concentrations of Sch B (0, 10, 20, 40, 80, 160 µmol/l). (E) Hoechst immunofluorescence staining of p65 expression in CCA cells. (F) CCA cell activity was assessed after treatment with Sch B and Bay 11–7082, a targeted NF-κB inhibitor. Statistical analysis was performed using ANOVA and Dunnett's post hoc test. *P<0.05, **P<0.01, ***P<0.001. Sch B, Schisandrin B; CCA, cholangiocarcinoma.

Article Snippet: p-p65 NF-κB (Ser536) , 3033 , 1:1,000 , Cell Signaling Technology, Inc..

Techniques: Expressing, Luciferase, Immunofluorescence, Staining, Activity Assay

Journal: Frontiers in Pharmacology

Article Title: Investigating the therapeutic mechanism of Puerarin in vascular dementia: an integrated approach combining network pharmacology and experimental validation

doi: 10.3389/fphar.2026.1796295

Figure Lengend Snippet: PUE inhibits the TLR4/MyD88/NF-κB signaling pathway in VaD rats. (A) mRNA levels of TLR4 and MyD88( n = 4); (B) Immunohistochemical staining of P- NF-κB P65 in hippocampus (200×, 400×); (C–F) Protein levels of TLR4, Myd88, P- NF-κB P65, NF-κB P65 ( n = 4); Data are presented as mean ± SEM. Significant differences compared with Sham group were designated as * P < 0.05, with Model group as # P < 0.05, with PUE group as & P < 0.05 and with LPS group as △ P < 0.05.

Article Snippet: Briefly, the sections were pretreated with sodium citrate buffer (Boster Biological Technology, Pleasanton, CA, USA, Cat. No.: AR0024) at 98 °C for 30 min for antigen retrieval, and then the endogenous peroxidase activity was blocked with SignalStain® Peroxidase Blocking Reagent (CST, Danvers, MA, USA, Cat. No.: 15039S) for 10 min and then with BSA (Biyuntian biological technology, Shanghai, China, Cat. No.: ST023) for 30 min, incubated with the primary P-NF-κB p65 (1:300, abmart, Shanghai, China, No.: TP56372) overnight at 4 °C, and finally with goat anti-mouse biotinylated antibody (1:200, CST, Danvers, MA, USA, No.: 14709S) for 30 min at room temperature.

Techniques: Immunohistochemical staining, Staining